Kinetics Studies of the Bleaching of Food Dyes >> Step 4: Putting it all together
Kinetics Studies of the Bleaching of Food Dyes
Step 4. Converting absorbance to concentration.
In order to assemble the rate law, Rate = k [dye]m[bleach]n, we need to convert the absorbance values to concentrations. To do this, we will use Beer's law, which may be written as:
abs = ε l [dye]
where l is the path length of light through the solution and ε is the molar absorptivity. For this experiment, we want to know the product, ε * l, since this is the proportionality constant between our measured absorbance and the dye concentration.
To determine the proportionality constant, we will perform a Beer's law calibration by measuring the abs for various dye concentrations. We will then construction a Beer's law plot with abs on the y-axis and concentration on the x-axis. The slope of this line is the porportionality constant, εl. With this proportionality constant, we will be able to determine the concentration for any absorbance value we collected in Steps 1 and 3.
Preparing a set of dilutions of the standard dye
To create a Beer's law plot, we will first prepare a set of dilutions of the standard Yellow 6 dye (using the tutor below) and then run an absorbance measurement of each solution. The dye solution has a concentration of 3.40E-5 M. Complete the following table, which prepares dilutions of the original dye solutions, each with a total volume of 10.00 mL.
Use the simulation below to collect the absorbance vs % dilution data for the Yellow 6 dye solutions.
Use the results of the above simulation to answer the following question:
Please use this information to complete the section of the homework document corresponding to step 4.
This concludes the online tutorial, please be sure to complete the activity form.