Kinetics ActivityStep 1: Making a kinetic trace Step 2a: Getting information from a kinetic trace Step 2b: Determining the pseudo rate constant k' Step 3: Determine the effect of bleach on the reaction Step 4: Converting absorbance to concentration 
Kinetics Studies of the Bleaching of Food Dyes >> Step 4: Putting it all together
Kinetics Studies of the Bleaching of Food DyesStep 4. Converting absorbance to concentration.In order to assemble the rate law, Rate = k [dye]^{m}[bleach]^{n}, we need to convert the absorbance values to concentrations. To do this, we will use Beer's law, which may be written as: abs = ε l [dye] where l is the path length of light through the solution and ε is the molar absorptivity. For this experiment, we want to know the product, ε * l, since this is the proportionality constant between our measured absorbance and the dye concentration. To determine the proportionality constant, we will perform a Beer's law calibration by measuring the abs for various dye concentrations. We will then construction a Beer's law plot with abs on the yaxis and concentration on the xaxis. The slope of this line is the porportionality constant, εl. With this proportionality constant, we will be able to determine the concentration for any absorbance value we collected in Steps 1 and 3. Preparing a set of dilutions of the standard dyeTo create a Beer's law plot, we will first prepare a set of dilutions of the standard Yellow 6 dye (using the tutor below) and then run an absorbance measurement of each solution. The dye solution has a concentration of 3.40E5 M. Complete the following table, which prepares dilutions of the original dye solutions, each with a total volume of 10.00 mL.
Use the simulation below to collect the absorbance vs % dilution data for the Yellow 6 dye solutions.
Use the results of the above simulation to answer the following question:
Please use this information to complete the section of the homework document corresponding to step 4. This concludes the online tutorial, please be sure to complete the activity form.
