Quantitative Analysis
Unknown DNA Solution Problem

A lot of research is being done on molecules that bind to DNA. The figure above shows one common binding mode, in which the molecule sticks into a groove of DNA. The binding is especially interesting if it is "sequence specific," such that the molecule binds only to specific sequences of DNA base pairs. Such molecules can be used as diagnostics, to indicate if a certain type of DNA is present in a sample. Often the molecule will change color when it binds, and this makes it easy to identify genes associated with certain viral infections. Another use of molecules that bind to DNA is to create drugs that bind to specific genes and prevent them from being expressed. You work for new startup biotechnology company. They have invented a new molecular dye, code-named Dyea, that binds selectively to a portion of viral DNA that we will can DNAa. The Dye is red in it's unbound form, but is clear (transparent, or white from the perspective of the virtual lab) when it's in it's bound form. Dyea reacts with DNAa according to the reaction,
| Dyea | + | DNAa | <==> | Dyea-DNAa
Color: | Red |  | Uncolored |  | Uncolored
Where Dyea-DNAa means a single molecule consisting of Dyea bound to DNAa One day, amidst your scramble to get to a meeting on time, you accidentally left a solution of DNAa on your workbench, and due to your short memory span you don't remember the concentration of this solution. You will find this solution and a 1.00 µM solution of Dyea in the stockroom. Use the virtual lab to experimentally determine the concentration of the solution of DNAa. Note that in this problem, you will not be able to use any of the solution viewers to read the concentrations of the solutions. Please briefly describe your approach and give your result.